无瓣海桑对乡土红树植物的化感作用

以外来种无瓣海桑为供体,通过室内栽培实验探讨了无瓣海桑各器官水浸液的不同浓度(即0 1、0 2、0 3、0 4、0 5g·mL-1)对我国4个乡土红树植物的化感作用。结果表明:无瓣海桑对各乡土红树植物均存在化感作用,对胚轴萌发的影响大于对幼苗生长的影响;除最低浓度外,不同浓度的各器官水浸液均能明显抑制各乡土红树植物的萌发,且随着浓度增大抑制作用增强;所有水浸液均表现出高浓度下抑制幼苗生长,随着浓度的降低抑制作用减弱,甚至转为促进的规律;各器官的水浸液对各乡土红树植物的萌发和幼苗生长的抑制作用为:果>根>凋落叶;高浓度时,无瓣海桑的各器官水浸液对不同受体红树植物的幼苗生长抑制强度不同。     

PGPB对红树植物木榄幼苗的接种效应

通过盆栽试验研究了植物促生菌(PGPB)对红树植物木榄幼苗的接种效应,供试菌为6种解磷菌(即B.amy,Vib,B.atr,Xan,B.Lic,P.M.)和5种固氮菌(即Au4,Phy,24S,JA4,cd),结果表明:(1)分别接种6种解磷菌株6个月后木榄幼苗的生长均得到了不同程度的促进,与对照相比,木榄苗高增加21.57%～9.54%,生物量增加27.49%～20.32%,叶片的氮含量增加33.33%～1.35%,叶片的磷含量增加24.42%～2.04%;经差异显著性检验,B.amy和Vib 2个菌种对木榄幼苗的促生效应比其它解磷菌株更明显;(2)分别接种5种固氮菌株6个月后木榄幼苗的生长均得到了促进,与对照相比,木榄幼苗的均高增加24.07%～10.22%,生物量增加32.36%～19.71%,叶片的氮含量增加23.39%～4.05%,叶片的磷含量增加32.79%～4.99%;差异显著性检验表明:Au4和Phy 2个菌种对木榄幼苗的促生效果比其它固氮菌更显著.     

应用PCR-DGGE技术比较绵羊瘤胃、网胃、瓣胃和皱胃菌群的多样性

本研究采用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)技术结合DGGE图谱条带的克隆和测序比较了绵羊瘤胃、网胃、瓣胃和皱胃菌群的多样性,同时对菌群进行了聚类分析和主成分分析。结果表明:绵羊瘤胃、网胃、瓣胃和皱胃内容物DGGE图谱的平均条带数分别为18、13、16和15条;瘤胃和瓣胃内菌群的多样性指数、均匀度和丰富度均较高,分别为2.83、0.79、17.00和2.82、0.79、16.80,而网胃和皱胃内容物较低,分别为2.52、0.70、12.60和2.73、0.76、15.40;聚类分析结果显示不同胃的内容物菌群具有一定差异,但不同动物个体相同胃的内容物相似性系数均高于0.63;PCR-DGGE图谱中测序的条带大多数归为拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes),优势菌群为厚壁菌门(Firmicutes)细菌、拟杆菌门(Bacteroidetes)细菌、未培养瘤胃细菌(uncultured rumen bacterium)、未培养细菌(uncultured bacterium)和韦荣球菌科(Veillonellaceae)细菌,特异性细菌为不动杆菌属(Acinetobacter sp.)细菌和瘤胃球菌属(Ruminococcaceae)细菌。结果提示,绵羊4个胃中均含有种类丰富和数量巨大的细菌,且随着消化道部位由前往后的顺序,菌群的多样性呈现先高后降低再升高的趋势。     

竹鼠肠道中厌氧纤维素降解菌的分离与鉴定

本试验旨在从竹鼠肠道中分离厌氧纤维素降解菌并鉴定。取中华竹鼠盲肠内容物稀释,采用含2种不同纤维素[微晶纤维素(MCC)和羧甲基纤维素钠(CMC-Na)]的培养基在厌氧条件下对纤维素降解菌进行初筛,后又经以纤维二糖为唯一碳源的培养基的多次复筛,选出的菌株进行经形态学和分子生物学的鉴定。结果表明:经过初筛和复筛,筛选出了2株厌氧纤维素降解菌。初筛培养基采用MCC和CMC-Na最终筛选出的2株纤维素降解菌的纤维素酶活力分别为6.599和5.268U/m L。菌株经PCR鉴定、测序,用所得序列构建系统发育树,得出这2株菌与酪酸梭状芽孢杆菌(Clostridium butyricum)具有很高的同源性,达99%,二者均属于梭菌属(Clostridium)。本试验从竹鼠盲肠中成功筛选出2株纤维素降解菌,经鉴定确定属于梭菌属。     

Tooth brushing inhibits oral bacteria in dogs

In this study, scaling, polishing and daily tooth brushing were performed in 20 beagle dogs, and the number of oral bacteria was determined using a bacterial counter. The dogs were randomized into the scaling (S), scaling + polishing (SP), scaling + tooth daily brushing (SB) and scaling + polishing + tooth daily brushing (SPB) groups. Samples were collected from the buccal surface of the maxillary fourth premolars of the dogs immediately after scaling and every week thereafter from weeks 1 to 8. Throughout the study, the number of bacteria was significantly lower in the SB and SPB groups compared with the S group. The findings suggest that daily tooth brushing inhibited oral bacterial growth in the dogs.     

Effect of lactoferrin on murine embryo development created from lipopolysaccharide-treated sperm

The effect of lactoferrin (LF) on embryo development was investigated by using lipopolysaccharide (LPS)-treated mouse sperm. For the development rate of the 2-cell stage embryo, the embryo derived from LPS- and LF-treated sperm showed similar survival rate to the control embryo. On day 12 after the embryo transfer into the recipient, the frequent abnormality was observed in the embryo derived from LPS-treated sperm, and the abnormality was tended to be inhibited in the embryo derived from LPS- and LF-treated sperm. These results imply that LF treatment on sperm contaminated with bacteria may facilitate the embryo development, which contribute to the improvement of infertility.     

Changes in the predicted function of the rumen bacterial community of Japanese Black beef cattle during the fattening stages according to Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses

We investigated changes in the predicted functions of the rumen bacterial community in Japanese Black beef cattle during fattening. Nine cattle were fed a high-concentrate diet during the early, middle, and late fattening stages consecutively (10–14, 15–22, and 23–30 months of age, respectively). The rumen fluid and solid samples collected at each stage were subjected to sequencing analyses. The sequencing results were clustered and classified into operational taxonomic units (OTUs). Representative sequences and a raw counting table for each OTU were submitted to the Piphillin website. The predicted functions were revealed by the Kyoto Encyclopedia of Genes and Genomes database as the ratio of the total sequence. In the early stage, “Biosynthesis of secondary metabolites” was significantly higher in the fluid fraction than in the solid fraction. “Two-component system” in the middle stage was significantly lower and “Purine metabolism” in the late stage was significantly higher in the fluid fraction than those in the solid fraction. The fluid fraction was significantly correlated with acetic acid, propionic acid, and bacterial metabolism, such as “Biosynthesis of secondary metabolites” and “Sugar metabolism.” Moreover, the solid fraction was correlated with “Purine metabolism” and “Biosynthesis of secondary metabolism”. These results suggest that the rumen bacterial community in Japanese Black beef cattle adapts to changes in rumen conditions by altering their functions in response to a long-term high-grain diet.     

1株具有免疫增强功能的乳酸菌筛选及其免疫效果评价

为筛选出具有较强免疫功能的益生菌用于临床研究,对分离自健康鸡粪便样品中的20株乳酸菌进行耐酸、耐胆盐、体外抑菌、黏附、胞外多糖含量测定试验以及小鼠免疫功能检测,并结合上述试验结果进行综合性评价.结果:筛选出20号菌株为目的菌株,耐酸、耐胆盐试验表明,该菌株在pH=2时的存活率为72.7％,在胆盐浓度为1.0％的环境中存...     

Molecular structure of endotoxins from Gram-negative marine bacteria: an update

Marine bacteria are microrganisms that have adapted, through millions of years, to survival in environments often characterized by one or more extreme physical or chemical parameters, namely pressure, temperature and salinity. The main interest in the research on marine bacteria is due to their ability to produce several biologically active molecules, such as antibiotics, toxins and antitoxins, antitumor and antimicrobial agents. Nonetheless, lipopolysaccharides (LPSs), or their portions, from Gram-negative marine bacteria, have often shown low virulence, and represent potential candidates in the development of drugs to prevent septic shock. Besides, the molecular architecture of such molecules is related to the possibility of thriving in marine habitats, shielding the cell from the disrupting action of natural stress factors. Over the last few years, the depiction of a variety of structures of lipids A, core oligosaccharides and O-specific polysaccharides from LPSs of marine microrganisms has been given. In particular, here we will examine the most recently encountered structures for bacteria belonging to the genera Shewanella, Pseudoalteromonas and Alteromonas, of the γ-Proteobacteria phylum, and to the genera Flavobacterium, Cellulophaga, Arenibacter and Chryseobacterium, of the Cytophaga-Flavobacterium-Bacteroides phylum. Particular attention will be paid to the chemical features expressed by these structures (characteristic monosaccharides, non-glycidic appendages, phosphate groups), to the typifying traits of LPSs from marine bacteria and to the possible correlation existing between such features and the adaptation, over years, of bacteria to marine environments.     

Osteomalacia as a result of phosphorus deficiency in beef cattle grazing subtropical native pastures in Uruguay

We investigated 2 outbreaks of osteomalacia as a result of phosphorus (P) deficiency in herds of lactating beef cows grazing subtropical native pastures in Uruguay. Cows exhibited pica, difficulty to stand and walk, rib fractures, and body weight loss even with adequate forage availability. Osteopenia and severe osteomalacia were observed on gross and histologic examination. The concentrations of bicarbonate-extractable P in soil (4.0, 4.1 mg P/kg), total P in pasture (0.9, 1.1 g P/kg), inorganic P in serum (1.0, 0.71 mmol P/L), and P in bone (73 mg P/mL) were all low. Although injectable and mineral salt supplements provided additional P in both outbreaks, these supplementary amounts were insufficient to prevent P deficiency. The P ingested by the cows from the pasture and supplements would have provided 20–55% of their daily P requirements of ~21 g P/d. Osteomalacia occurred in cattle at the 2 ranches as a result of severe P deficiency in the soil and forage, and inadequate P supplementation. Following diagnosis, control of P deficiency in beef cattle requires estimation of the amount of pasture P ingested and provision of sufficient additional supplementary P to meet the animals’ requirements.